Reducing WBC background in cancer cell separation products

SweCRIS

Along with high separation efficiency, the cancer cell recovery (input to output) displayed an average MCF-7 recovery of 89.8 ± 5.3% in the center fraction (Fig. 5c). 11. Acoustophoresis cell separation and sorting technique.

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US10640760B2 - Therapeutic cell washing, concentration, and separation utilizing acoustophoresis - Google Patents Therapeutic cell washing, concentration, and separation utilizing acoustophoresis Download PDF Info Publication number US10640760B2. Novel Cell Sorting and Separation Market: Focus on Acoustophoresis, Buoyancy-activated, Dielectrophoresis, Magnetophoretics, Microfluidics, Optoelectronics, … “Microfluidic acoustic separation (or acoustophoresis) is gentle on cells, has high selectivity that responds to cell size, density and compressibility and can exceed the capabilities of centrifugation or filtration,” said Fiering. Reducing WBC background in cancer cell separation products by negative acoustic contrast particle immuno-acoustophoresis Kevin Cushing a,1, Eva Undvall a,1, Yvonne Ceder b, Hans Lilja c, d, e On the basis of these findings, we anticipated that the separation of cancer cells from blood would be greatly improved with regard to WBCs, compared to previously reported acoustophoresis-based methods of cell separation. 21 A dramatic improvement was indeed seen, yielding tumor cell recoveries of 87 and 83% of fixed tumor cells (DU145 and PC3, respectively) and a corresponding WBC For cells fixed with paraformaldehyde, cancer cell recovery ranged from 93.6% to 97.9% with purity ranging from 97.4% to 98.4%. There was no detectable loss of cell viability or cell proliferation subsequent to the exposure of viable tumor cells to acoustophoresis. For nonfixed, viable cells, tumor cell Global Novel Cell Sorting and Separation Market Report 2019-2030: Focus on Acoustophoresis, Buoyancy, Dielectrophoresis, Magnetophoretics, Microfluidics, Optoelectronics, Traceless Affinity Acoustophoresis is a technique that applies ultrasonic standing wave forces in a microchannel to sort cells depending on their physical properties in relation to the surrounding media.

Label-free neuroblastoma cell separation from hematopoietic progenitor cell products using acoustophoresis – towards cell processing of complex biological samples. Franziska Olm, Anke Urbansky, Josefina H. Dykes, Thomas Laurell, and Stefan Scheding. US10640760B2 - Therapeutic cell washing, concentration, and separation utilizing acoustophoresis - Google Patents Therapeutic cell washing, concentration, and separation utilizing acoustophoresis Download PDF Info Publication number US10640760B2.

‪Thomas Laurell‬ - ‪Google Scholar‬

“On microchannel acoustophoresis – Experimental considerations and life science “Metabolic Studies with Liquid Separation Coupled to Mass Spectrometry”, Erik  med hjälp av metoden som kallas akustisk cellseparation. Progenitor Cell Products Using a Novel Micro-Chip Based Acoustophoretic  Ann-Margret, Åberg, Lena, Ohlin, Mats och Laurell, Thomas, Cell separation based on acoustophoresis and applications in health care, 2010.

Acoustofluidics papers Biomedical Engineering

10 Nov 2019 spheres sorted by acoustophoresis with in-line resistive pulse sensing. Physical a separation distance of a quarter-wavelength. In order.

Biomedical Engineering, Dongguk University, Seoul, SOUTH KOREA ABSTRACT The basic principles of acoustic cell and particle manipulation in chip integrated acoustic standing … 2018-09-19 A novel method, free flow acoustophoresis (FFA), capable of continuous separation of mixed particle suspensions into multiple outlet fractions is presented. Acoustic forces are utilized to separate particles based on their size and density. The method is shown to be suitable for both biological and nonbiological suspended particles. Using this microchannel configuration, several publications have shown cancer cell lines (breast and prostate) being efficiently separated from WBCs from RBC-lysed blood using acoustophoresis with BASWs [3, 20].This label-free separation occurs because larger-sized cancer cells focus faster to the center pressure node than WBCs in the separation channel; thus, if flow rates are optimized the Acoustophoresis enables the label-free separation of functionally different subsets of cultured bone marrow stromal cells Franziska Olm , Hooi Ching Lim , Katharina Schallmoser , Dirk Strunk , Thomas Laurell & Stefan Scheding , 2020 Jun 15 , In : Cytometry. 2018-09-01 2017-12-07 Immunomagnetic cell separation is a technique whereby magnetic particles are used to isolate target cells from heterogeneous mixtures.
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Acoustophoresis cell separation and sorting technique. Acoustic cell separation and sorting techniques rely on the fact that cells and particles suspended in fluid experience an acoustic radiation force when they are exposed to ultrasound. The acoustophoresis separation device is a silicon/glass microchannel [7] having a trifurcation inlet and outlet, Fig. 1. A piezoceramic crystal actuates the separation channel at /2 resonance, creating a force on suspended cells directed to-wards the vertical center plane of the channel.

Binary separation of particle/vesicle/cell mixtures into two subpopulations is outlined based on the different polarities of the acoustic contrast factor.
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Publications within Engineering Sciences - Centre of Natural

MSC products are heterogeneous and we therefore investigated whether acoustophoresis, an ultrasound‐based separation technology, could be used for the label‐free enrichment of functionally different MSC populations. 2017-12-07 Article I: Label-free neuroblastoma cell separation from hematopoietic progenitor cell products using acoustophoresis - towards cell processing of complex biological samples Article II: Label-free separation of neuroblastoma patient-derived xenograft (PDX) cells from hematopoietic progenitor cell products by acoustophoresis Article III: Acoustophoresis enables label-free separation of functionally different Petersson, F., et al.: Free flow acoustophoresis: Microfluidic-based mode of particle and cell separation. Anal. Chem.